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Polarized Light Spectroscopy for Measurement of the Microvascular Response to Local Heating at Multiple Skin Sites
Author(s) -
TESSELAAR ERIK,
BERGKVIST MAX,
SJÖBERG FOLKE,
FARNEBO SIMON
Publication year - 2012
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/j.1549-8719.2012.00203.x
Subject(s) - microcirculation , reproducibility , laser doppler velocimetry , skin temperature , spectroscopy , perfusion , materials science , chemistry , biomedical engineering , nuclear medicine , medicine , analytical chemistry (journal) , blood flow , chromatography , physics , quantum mechanics
Please cite this paper as: Tesselaar E, Bergkvist M, Sjöberg F, Farnebo S. Polarized light spectroscopy for measurement of the microvascular response to local heating at multiple skin sites. Microcirculation 19 : 705–713, 2012. Abstract Objective: To evaluate whether TiVi, a technique based on polarized light, could measure the change in RBC concentration during local heating in healthy volunteers. Methods: Using a custom‐made transparent heater, forearm skin was heated to 42 °C for 40 minutes while the change in RBC concentration was measured with TiVi. The perfusion response during local heating was measured at the same time with Laser Doppler flowmetry. Results: Mean RBC concentration increased (91 ± 34 vs. 51 ± 34 A.U. at baseline, p < 0.001). The spatial heterogeneity of the RBC concentration in the measured skin areas was 26 ± 6.4% at baseline, and 23 ± 4.6% after 40 minutes of heating. The mean RBC concentrations in two skin sites were highly correlated (0.98 at baseline and 0.96 after 40 minutes of heating). The change in RBC concentration was less than the change in perfusion, measured with LDF. Unlike with LDF, a neurally mediated peak was not observed with TiVi in most of the test subjects. Conclusions: TiVi is a valuable technique for measuring the microvascular response to local heating in the skin, and offers a high reproducibility for simultaneous measurements at different skin sites, provided carefully controlled experiments are ensured.