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A Human Vascular Model Based on Microdialysis for the Assessment of the Vasoconstrictive Dose–Response Effects of Norepinephrine and Vasopressin in Skin
Author(s) -
FOLKESSON KIM TCHOU,
SAMUELSSON ANDERS,
TESSELAAR ERIK,
DAHLSTRÖM BENGT,
SJÖBERG FOLKE
Publication year - 2012
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/j.1549-8719.2012.00170.x
Subject(s) - microdialysis , vasopressin , vasoconstriction , norepinephrine , medicine , endocrinology , urea , chemistry , biochemistry , dopamine , central nervous system
Please cite this paper as : Folkesson KT, Samuelsson A, Tesselaar E, Dahlström B, Sjöberg F. A human vascular model based on microdialysis for the assessment of the vasoconstrictive dose–response effects of norepinephrine and vasopressin in skin. Microcirculation 19: 352–359, 2012. Abstract Objective: Microdialysis enables drug delivery in the skin and simultaneous measurement of their effects. The present study aimed to evaluate dose‐dependent changes in blood flow and metabolism during microdialysis of norepinephrine and vasopressin. Methods: We investigated whether increasing concentrations of norepinephrine (NE, 1.8–59 μmol/L) and vasopressin (VP, 1–100 nmol/L), delivered sequentially in one catheter or simultaneously through four catheters, yield dose‐dependent changes in blood flow (as measured using urea clearance) and metabolism (glucose and lactate). Results: We found a significant dose‐dependent vasoconstriction with both drugs. Responses were characterized by a sigmoid dose response model. Urea in the dialysate increased from a baseline of 7.9 ± 1.7 to 10.9 ± 0.9 mmol/L for the highest concentration of NE ( p < 0.001) and from 8.1 ± 1.4 to 10.0 ± 1.7 mmol/L for the highest concentration of VP ( p = 0.037). Glucose decreased from 2.3 ± 0.7 to 0.41 ± 0.18 mmol/L for NE ( p = 0.001) and from 2.7 ± 0.6 to 1.3 ± 0.5 mmol/L for VP ( p < 0.001). Lactate increased from 1.1 ± 0.4 to 2.6 ± 0.5 mmol/L for NE ( p = 0.005) and from 1.1 ± 0.4 to 2.6 ± 0.5 mmol/L for VP ( p = 0.008). There were no significant differences between responses from a single catheter and from those obtained simultaneously using multiple catheters. Conclusions: Microdialysis in the skin, either with a single catheter or using multiple catheters, offers a useful tool for studying dose response effects of vasoactive drugs on local blood flow and metabolism without inducing any systemic effects.