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A Micro‐Delivery Approach for Studying Microvascular Responses to Localized Oxygen Delivery
Author(s) -
GHONAIM NOUR W.,
LAU LEO W. M.,
GOLDMAN DANIEL,
ELLIS CHRISTOPHER G.,
YANG JUN
Publication year - 2011
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/j.1549-8719.2011.00132.x
Subject(s) - oxygen delivery , microcirculation , oxygen , intravital microscopy , oxygen transport , biomedical engineering , microfabrication , in vivo , capillary action , biophysics , chemistry , anatomy , materials science , nanotechnology , medicine , biology , pathology , fabrication , alternative medicine , microbiology and biotechnology , organic chemistry , composite material
Please cite this paper as: Ghonaim, Lau, Goldman, Ellis, and Yang (2011). A Micro‐delivery Approach for Studying Microvascular Responses to Localized Oxygen Delivery. Microcirculation 18 (8), 646–654. Abstract Background: In vivo video microscopy has been used to study blood flow regulation as a function of varying oxygen concentration in microcirculatory networks. However, previous studies have measured the collective response of stimulating large areas of the microvascular network at the tissue surface. Objective: We aimed to limit the area being stimulated by controlling oxygen availability to highly localized regions of the microvascular bed within intact muscle. Design and Method: Gas of varying O 2 levels was delivered to specific locations on the surface of the Extensor Digitorum Longus muscle of rat through a set of micro‐outlets (100 μ m diameter) patterned in ultrathin glass using state‐of‐the‐art microfabrication techniques. O 2 levels were oscillated and digitized video sequences were processed for changes in capillary hemodynamics and erythrocyte O 2 saturation. Results and Conclusions: Oxygen saturations in capillaries positioned directly above the micro‐outlets were closely associated with the controlled local O 2 oscillations. Radial diffusion from the micro‐outlet is limited to ∼75 μ m from the center as predicted by computational modeling and as measured in vivo . These results delineate a key step in the design of a novel micro‐delivery device for controlled oxygen delivery to the microvasculature to understand the fundamental mechanisms of microvascular regulation of O 2 supply.