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Glycine and Taurine Equally Prevent Fatty Livers from Kupffer Cell‐Dependent Injury: An In Vivo Microscopy Study
Author(s) -
BRUNS HELGE,
WATANPOUR INGO,
GEBHARD MARTHAMARIA,
FLECHTENMACHER CHRISTA,
GALLI UWE,
SCHULZEBERGKAMEN HENNING,
ZORN MARKUS,
BÜCHLER MARKUS W.,
SCHEMMER PETER
Publication year - 2011
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/j.1549-8719.2010.00078.x
Subject(s) - taurine , kupffer cell , glycine , steatosis , in vivo , fatty liver , liver injury , ex vivo , medicine , endocrinology , reperfusion injury , andrology , chemistry , ischemia , pathology , biology , biochemistry , microbiology and biotechnology , disease , amino acid
Please cite this paper as : Bruns, Watanpour, Gebhard, Flechtenmacher, Galli, Schulze‐Bergkamen, Zorn, Büchler and Schemmer (2011). Glycine and Taurine Equally Prevent Fatty Livers from Kupffer Cell‐Dependent Injury: An In Vivo Microscopy Study. Microcirculation 18 (3), 205–213. Abstract Background: IRI still is a major problem in liver surgery due to warm ischemia and organ manipulation. Steatosis is not only induced by diabetes, hyperalimentation, alcohol and toxins, but also chemotherapy given before resection. Since steatotic livers are prone to Kupffer cell‐dependent IRI, protection of steatotic livers is of special interest. This study was designed to compare the effect of taurine and glycine on IRI in steatotic livers. Materials and Methods: Steatosis was induced with ethanol (7 g/kg b.w.; p.o.) in female SD rats. Ten minutes after inactivation of Kupffer cells with taurine or glycine (300 mM; i.v.), left liver lobes underwent 60 minutes of warm ischemia. Controls received the same volume of valine (300 mM; i.v.) or normal saline. After reperfusion, white blood cell‐endothelial interactions and latex‐bead phagocytosis by Kupffer cells were investigated. Liver enzymes were measured to estimate injury. For statistical analysis, ANOVA and Student’s t ‐test were used. Results: Glycine and taurine significantly decreased leukocyte‐ and platelet‐endothelium interactions and latex‐bead phagocytosis ( p < 0.05). Liver enzymes were significantly lower after glycine and taurine ( p < 0.05). Conclusions: This study shows that preconditioning with taurine or glycine is equally effective in preventing injury to fatty livers most likely via Kupffer cell‐dependent mechanisms.