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Expression of Endothelial Cell Adhesion Molecules in Neovascularized Tissue
Author(s) -
VALLIEN GINA,
LANGLEY ROBERT,
JENNINGS STEPHEN,
SPECIAN ROBERT,
GRANGER D. NEIL
Publication year - 2000
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/j.1549-8719.2000.tb00125.x
Subject(s) - cell adhesion molecule , microbiology and biotechnology , cell adhesion , chemistry , adhesion , cell , biology , biochemistry , organic chemistry
Objective : Recent studies indicate that endothelial cells of newly formed blood vessels are activated and exhibit a distinct phenotype that may influence the responses of these microvessels to an inflammatory stimulus. The objective of this study was to compare the basal and cytokine‐stimulated expression of endothelial cell adhesion molecules in neovascularized tissue to normal (nonproliferating) vascular beds. Methods : The expression of P‐ and E‐selectin, VCAM‐1, ICAM‐1, ICAM‐2, and PECAM‐1 was measured, using the dual radiolabeled mAb technique, in subcutaneously implanted (for 10–15 days) polyurethane sponges, skin, heart, lung, and intestine of male C57BL/6 mice (background). Results : Basal values of PECAM‐1 and ICAM‐2 revealed a low vascular density in the implanted sponge matrices that is comparable to skin. When normalized for vascular surface area (PECAM‐1 or ICAM‐1 expression), the basal level of E‐ and P‐selectin expression was highest in neovascularized sponge and skin. TNF‐α elicited an increased expression of all endothelial CAMs, except PECAM‐1 and ICAM‐2, but the responses were blunted in sponge and skin, relative to other vascular beds. Conclusions : These findings indicate that endothelial cells in newly formed blood vessels exhibit a pattern of basal and cytokine‐induced expression of certain adhesion glycoproteins that is similar to nonproliferating cutaneous vessels.