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Life and Death in the Microcirculation: a Role for Angiotensin II
Author(s) -
GREENE ANDREW S.
Publication year - 1998
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.1111/j.1549-8719.1998.tb00060.x
Subject(s) - microcirculation , angiotensin ii , microvessel , endocrinology , medicine , renin–angiotensin system , receptor , biology , immunohistochemistry , blood pressure
Objective : Angoitensin II (ANGII) plays a critical role in the maintenance of the microcirculation and in the anatomical loss of microvessels (rarefaction) that occurs in low renin forms of hypertension and in animals fed a high‐salt diet. Elevations in sodium intake can trigger a series of hemodynamic and hormonal responses culminating in a substantial rarefaction of small arterioles and capillaries in both normal and reduced renal mass hypertensive rats. Methods : Immunohistochemistry, Northern blot, and reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of microdissected blood vessels were used to localize ANGII receptors in the microcirculation. Chronic infusion of ANGII and other physiologic and pharmacologic manipulations of the renin‐angiotensin system in rats was combined with morphologic and mathematical analysis of the network architecture. Results : We have shown that rarefaction of the microcirculation can cause an increase in total peripheral resistance, reduced tissue perfusion, decreased oxygen delivery, and impaired organ function. Although the mechanisms by which this occurs are not well understood, a number of key observations point to a role for the renin‐angiotensin system in this effect. First, ANGII infused systemically at subpressor levels, or locally into the skeletal muscle interstitium, can induce significant microvessel growth. Second, localization of ANGII receptor proteins by immunohistochemistry and Western blotting and RNA localization by RT‐PCR confirm the presence of AT 1 receptors, which are growth‐stimulatory, and AT 2 receptors, which are growth‐inhibitory in the microcirculation. Third, maintenance of ANGII at normal levels during periods of hypertension or high‐salt diet completely eliminates rarefaction. Conclusions : Taken together, these results support the hypothesis that ANGII acting through AT 1 ‐ and AT 2 ‐receptor mechanisms modulate vessel density during high‐salt diet and hypertension.