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Inhibitory effect of PPAR‐Gamma activator on IL‐6 and mPGES protein expression in PBMC induced by homocysteine
Author(s) -
Peng Youming,
Liu Hong,
Liu Fuyou,
Wang Haitao,
Liu Yinghong,
Duan Shaobin
Publication year - 2005
Publication title -
hemodialysis international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.658
H-Index - 47
eISSN - 1542-4758
pISSN - 1492-7535
DOI - 10.1111/j.1542-4758.2005.01165.x
Subject(s) - peripheral blood mononuclear cell , homocysteine , activator (genetics) , troglitazone , peroxisome proliferator activated receptor , endocrinology , medicine , rosiglitazone , receptor , in vitro , chemistry , biochemistry
The objective was to investigate the relationship between homocysteine (HCY), peroxisome proliferator‐activated receptors (PPAR)‐γ and the expression of interleukin 6 (IL‐6) and microsomal prostaglandin E synthase (mPGES) by peripheral blood mononuclear cell (PBMC) culture in vitro, as well as to look at the intervention with HCY and PPAR‐γ activators (troglitazone and rosiglitazone). PBMC were isolated from venous blood of normal volunteers and incubated with HCY and PPAR‐γ activator of varying concentrations for 24 hr. The supernatant of the culture medium was collected in order to determine the IL‐6 and mPGES levels by ELISA assay. The results show that the expression of IL‐6 and mPGES in the HCY experimental group was significantly higher than that in the control group (p<0.01 and p<0.05, respectively). Additionally, the IL‐6 and mPGES levels rose with the increase of HCY in a dose‐dependent manner (p<0.01 and p<0.05, respectively). PPAR‐γ activator could significantly inhibit the increase of IL‐6 and mPGES induced by 100 μmol/L HCY (p<0.05 and p<0.01, respectively). In conclusion, HCY can increase the expression of IL‐6 and mPGES, while PPAR‐γ activator can inhibit the increase of IL‐6 and mPGES induced by HCY.