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Myocardial Antioxidant Defenses During Cardiopulmonary Bypass
Author(s) -
Mezzetti Andrea,
Lapenna Domenico,
Pierdomenico Sante D.,
Giammarco Gabriele,
Bosco Giovanni,
Ilio Carmine,
Santarelli Paolo,
Calafiore Antonio M.,
Cuccurullo Franco
Publication year - 1993
Publication title -
journal of cardiac surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 58
eISSN - 1540-8191
pISSN - 0886-0440
DOI - 10.1111/j.1540-8191.1993.tb00368.x
Subject(s) - tbars , medicine , cardiopulmonary bypass , glutathione peroxidase , glutathione reductase , antioxidant , oxidative stress , glutathione , thiobarbituric acid , catalase , anesthesia , cardiology , biochemistry , lipid peroxidation , enzyme , chemistry
A bstract In 31 male patients undergoing coronary bypass surgery who underwent different periods of cardioplegic hypothermic arrest, the activities of glutathione peroxidase, glutathione reductase, glutathione transferase, copper/zinc‐containing and manganese‐containing super‐oxide dismutases, and catalase were studied in the right atrial myocardium, before and 5 minutes after aortic cross‐clamping. The levels of thiobarbituric acid reactive substances (TBARS) and nonproteic thiol compounds (NP‐SH) were also assessed. Prolonged ischemia followed by reperfusion induced activation of the major myocardial antioxidant enzymes with marked NP‐SH depression and TBARS increase, despite cold crystalloid cardioplegic protection. These changes were significantly related to the duration of the ischemic arrest, suggesting: (1) that reperfusion free radical generation is dependent on the severity of the previous ischemic period; and (2) the occurrence of myocardial oxidative stress during cardiopulmonary bypass.