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Effect of a Stabilized Microbubble Contrast Agent on CW Ultrasound Induced Red Blood Cell Lysis In Vitro
Author(s) -
MILLER M. W.,
AZADNIV M.,
DOIDA Y.,
BRAYMAN A. A.
Publication year - 1995
Publication title -
echocardiography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.404
H-Index - 62
eISSN - 1540-8175
pISSN - 0742-2822
DOI - 10.1111/j.1540-8175.1995.tb00515.x
Subject(s) - lysis , ultrasound , in vitro , chemistry , biomedical engineering , red blood cell , biophysics , medicine , radiology , biochemistry , biology
Human red blood cells (RBCs) in vitro at various fractional hematocrits (HCTs) were exposed for 60–120 seconds in a dialysis tubing vessel to 1 MHz continuous wave ultrasound (0–5 W/cm 2 SPTA intensity); exposure vessels were either rotated at 200 rpm or stationary. Some RBC suspensions also contained Albunex® (ALX; a commercially‐produced microbubble clinical ultrasound contrast agent) at final concentrations ranging from 0–41 μL/mL RBC suspension. Isonation was either by one transducer or by two opposing, continuously‐gated, balanced transducers. For the vessel rotation / no rotation experiments, ultrasound‐induced cell lysis was always increased with the ALX regimen relative to that of the no‐ALX regimen at HCTs up to about 10%; at higher HCTs (including whole blood), ultrasound‐induced hemol‐ysis essectially ceased to occur. The data are consistent with reports of the ineffectiveness of continuous wave ultrasound at 3–5 W/cm 2 to lyse cells in vitro at physiological cell densities with or without echocontrast medium supplemantation.