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High‐Resolution Optical Mapping of Ventricular Tachycardia in Rats with Chronic Myocardial Infarction
Author(s) -
DING CHUNHUA,
GEPSTEIN LIOR,
NGUYEN DUY THAI,
WILSON EMILY,
HULLEY GEORGE,
BEASER ANDREW,
LEE RANDALL J.,
OLGIN JEFFREY
Publication year - 2010
Publication title -
pacing and clinical electrophysiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.686
H-Index - 101
eISSN - 1540-8159
pISSN - 0147-8389
DOI - 10.1111/j.1540-8159.2010.02704.x
Subject(s) - medicine , cardiology , myocardial infarction , ventricular tachycardia , optical mapping , tachycardia
Background: Ventricular tachycardia (VT) is a common cause of mortality in post‐myocardial infarction (MI) patients, even in the current era of coronary revascularization treatment. We report a reproducible VT model in rats with chronic MI induced by ischemia‐reperfusion and describe its electrophysiological characteristics using high‐resolution optical mapping.Methods: An MI was generated by left anterior descending coronary ligation (25 minutes) followed by reperfusion in 20 rats. Electrophysiology study and optical mapping were performed 5 weeks later using a Langendorff‐perfused preparation and compared to normal rats.Results: The conduction velocity of the MI border zone was decreased to 53% of the normal areas remote from the infarct (0.37 ± 0.16 m/sec vs 0.70 ± 0.09 m/sec, P < 0.0001). The rate of VT inducibility in MI rats was significantly greater than in normal control rats (70% vs 0%, P = 0.00002). VT circuits involving the infarct area were identified with optical mapping in 83% MI rats. In addition, fixed and functional conduction block were observed in the infarct border zone.Conclusion: This ischemia‐reperfusion MI rat model is a reliable VT model, which simulates clinical revascularization treatment. High‐resolution optical mapping in this model is useful to study the mechanism of VT and evaluate the effects of therapies. (PACE 2010; 33:687–695)