Premium
Fluorescence spectroscopy in biochemistry: teaching basic principles with visual demonstrations
Author(s) -
Croney John C.,
Jameson David M.,
Learmonth Robert P.
Publication year - 2001
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1111/j.1539-3429.2001.tb00071.x
Subject(s) - fluorescence , curriculum , fluorescence spectroscopy , chemistry , computer science , engineering ethics , biophysics , engineering , biology , optics , psychology , physics , pedagogy
Although most biochemistry curricula include some treatment of light absorption and spectrophotometry, discussion of fluorescence spectroscopy is generally omitted. This omission is unfortunate given the increasing use of fluorescence in many fields of biochemical research. In this paper we briefly review the principles and applications of fluorescence in biochemical systems, from the viewpoint of teaching fluorescence in undergraduate curricula. Simple practical demonstrations are presented, which clearly demonstrate important concepts yet require minimal specialized equipment. © 2001IUBMB. Published by Elsevier Science Ltd. All rights reserved.