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An approach to the critical assessment of the experimental conditions in practical molecular biology: isolation of plant DNA
Author(s) -
Scarafoni Alessio,
Duranti Marcello
Publication year - 2001
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1111/j.1539-3429.2001.tb00058.x
Subject(s) - dna , computational biology , dna extraction , biochemical engineering , plant biology , biology , dna sequencing , set (abstract data type) , computer science , microbiology and biotechnology , biological system , process engineering , biochemistry , engineering , polymerase chain reaction , gene , botany , programming language
A plant molecular biology laboratory class experiment, based on real lab situations, is proposed to illustrate to the students how the assessment of the experimental conditions and the correct application of the technical details are critical also with an established procedure. During plant genomic DNA isolation and purification, a number of undesired effects, including DNA shearing and formation of secondary compounds, may take place. To simulate such events, we have set up a simple practical laboratory experiment in which the students are asked to isolate plant DNA under three different working conditions, from an optimal to a coarse one. The subsequent analyses on the extracted DNA enable the students to evaluate the influence of these different manipulations on DNA yield and quality. The experiment described can help the students to adopt a critical approach when interpreting the results of common preparative molecular techniques. © 2001 IUBMB. Published by Elsevier Science Ltd. All rights reserved.