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A simple method for immunodetection of membrane‐associated proteins
Author(s) -
Daghastanli Katia R.P.,
Ferreira Rinaldo B.,
Jr. Geraldo Thedei,
Ciancaglin Pietro
Publication year - 2000
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1111/j.1539-3429.2000.tb00164.x
Subject(s) - pasteurella multocida , fowl cholera , blot , antigen , immunodiffusion , membrane protein , microbiology and biotechnology , membrane , western blot , biology , bacterial outer membrane , chemistry , biochemistry , bacteria , immunology , escherichia coli , genetics , gene
The main objective of the present study was to identify membrane antigenic proteins using immunodiffusion and Western blotting techniques. This multidisciplinary experiment integrates biochemical principles with immunological and microbiological concepts. Pasteurella multocida , the causative agent of fowl cholera and other diseases like haemorrhagic septicaemia in many wild and domestic animals, was chosen as a biological model in order to obtain membrane antigenic proteins. We designed some simple laboratory experiments: partial purification of IgG, study of the effect of detergent on immunodiffusion, electrophoresis and Western blotting of membrane protein fraction of Pasteurella multocida . The results obtained with these techniques revealed that the membrane antigenic proteins Pasteurella multocida presented had M r 's of around 110, 104, 86, 71, 65 and 40kDa. © 2000 IUBMB. Published by Elsevier Science Ltd. All rights reserved.