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Capillary electrophoresis: theory, teaching approach and separation of oligosaccharides using indirect UV detection
Author(s) -
Lagane Bernard,
Treilhou Michel,
Couderc François
Publication year - 2000
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1111/j.1539-3429.2000.tb00163.x
Subject(s) - capillary electrophoresis , electrophoresis , chromatography , joule heating , chemistry , capillary action , field (mathematics) , joule (programming language) , analytical chemistry (journal) , materials science , physics , mathematics , thermodynamics , power (physics) , pure mathematics , composite material
Capillary electrophoresis, a recent analytical method (the first commercial instrument was sold just 10 years ago), offers an efficient alternative means compared to other current separation techniques. Due to a wide application range, this method is becoming more and more important among analytical laboratories. Thus, introductory lectures on this analytical technique are of particular interest. Moreover, the use of capillary electrophoresis aids in the understanding of the numerous parameters which influence electrophoretic migrations, such as ion mobilities, electrical field, pH, pK a , joule heating and buffering capacity. This article presents the theory of capillary electrophoresis and various steps of an analysis of four carbohydrates using this technique. This presentation of the data is similar to the way the students, to whom this analysis was asked, presented theirs. Representative results obtained by four students out of 100 are shown. © 2000 IUBMB. Published by Elsevier Science Ltd. All rights reserved.