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In vitro and in vivo proliferation, differentiation and migration of cardiac endothelial progenitor cells (SCA1 + /CD31 + side‐population cells)
Author(s) -
LIANG S. X.,
KHACHIGIAN L. M.,
AHMADI Z.,
YANG M.,
LIU S.,
CHONG B. H.
Publication year - 2011
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1111/j.1538-7836.2011.04375.x
Subject(s) - cd31 , progenitor cell , microbiology and biotechnology , population , stem cell , side population , in vivo , endothelial stem cell , biology , angiogenesis , endothelial progenitor cell , in vitro , cell growth , immunology , chemistry , cancer research , medicine , cancer stem cell , biochemistry , genetics , environmental health
To cite this article: Liang SX, Khachigian LM, Ahmadi Z, Yang M, Liu S, Chong BH. In vitro and in vivo proliferation, differentiation and migration of cardiac endothelial progenitor cells (SCA1 + /CD31 + side‐population cells). J Thromb Haemost 2011; 9 : 1628–37. Summary. Background: Side‐population (SP) cells are a select population identified by a capacity to efflux Hoechst dye and are enriched for stem/progenitor cell activity. Previous studies suggested that cardiac SP (CSP) cells could be divided into SCA1 + /CD31 − and SCA1 + /CD31 + CSP cells. SCA1 + /CD31 − CSP cells have been shown to be cardiac stem/progenitor cells. However, SCA1 + /CD31 + CSP cells have not been fully characterized. Objective: The aim of the present study was to characterize SCA1 + /CD31 + CSP cells in the adult mouse heart, and investigate their abilities to proliferate, differentiate, vascularize and migrate in vitro and in vivo . Results: Using fluorescence‐activated cell sorting (FACS), RT‐PCR, and assays of cell proliferation, differentiation and migration, and a murine model of myocardial infarction (MI), we showed that SCA1 + /CD31 + CSP cells express stem cell and endothelial‐specific genes, and reside in the blood vessels. These cells were able to proliferate, differentiate, migrate and vascularize in vitro and in vivo . After MI, SDF‐1α and CXCR4 were up‐regulated in the damaged myocardium and on SCA1 + /CD31 + CSP cells, respectively. Our results further showed that SDF‐1α induced migration of these cells in vitro . Importantly, we found that SCA1 + /CD31 + CSP cells could migrate into the ischemic region from the non‐ischemic area within the myocardium and form a vascular tube‐like structure after MI. Conclusions: Based on the gene expression profile, localization of SCA1 + /CD31 + CSP cells, and their ability to proliferate, differentiate, migrate and vascularize in vitro and in vivo , we postulate that SCA1 + /CD31 + CSP cells may represent endothelial progenitor cells in the mouse heart.