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Src family kinases are essential for primary aggregation by G i ‐coupled receptors
Author(s) -
NASH C. A.,
SÉVERIN S.,
DAWOOD B. B.,
MAKRIS M.,
MUMFORD A.,
WILDE J.,
SENIS Y. A.,
WATSON S. P.
Publication year - 2010
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1111/j.1538-7836.2010.03992.x
Subject(s) - kinase , proto oncogene tyrosine protein kinase src , src family kinase , chemistry , tyrosine protein kinase csk , p2y12 , receptor , platelet , signal transduction , adenylyl cyclase , platelet activation , medicine , sh3 domain , endocrinology , platelet aggregation , microbiology and biotechnology , biochemistry , biology
Summary. Introduction and Background: Adrenaline stimulates biphasic aggregation in plasma through the G i ‐coupled α 2A ‐adrenoreceptor. In the present study, we demonstrate that both primary and secondary wave aggregation induced by adrenaline in plasma is blocked by two structurally distinct inhibitors of Src family kinases, dasatinib and PD0173952. Methods and Results: In contrast, primary aggregation is partially inhibited or unaffected in the presence of inhibitors of cyclo‐oxygenase, phosphoinositide (PI) 3‐kinases, and P2Y 1 and P2Y 12 ADP receptors, although secondary aggregation is abolished. The ability of adrenaline to inhibit adenylyl cyclase and to synergize with platelet agonists in mediating platelet activation in plasma is retained in the presence of Src family kinase inhibition. Moreover, adrenaline does not activate Src family kinases, as determined by western blotting of their regulatory tyrosines, suggesting that constitutive signaling from Src family kinases may underlie their role in activation. Adrenaline is widely used in clinical laboratories for investigation of patients with suspected bleeding disorders. In a group of 90 unrelated patients with a clinically diagnosed platelet bleeding disorder, we identified four who did not exhibit primary wave aggregation in response to adrenaline, although the catecholamine potentiated the response to other agonists, and five who failed to undergo secondary wave aggregation. In contrast, adrenaline stimulated biphasic aggregation in 60 controls. All of the patients with a defective response to adrenaline had impaired ADP‐induced platelet activation. Conclusions: The present results indicate a previously unappreciated role for Src family kinases in mediating G i signaling in plasma, and demonstrate heterogeneity in response to adrenaline in patients with a clinically diagnosed platelet disorder.