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Proteolytic cleavage of factor VIII heavy chain is required to expose the binding‐site for low‐density lipoprotein receptor‐related protein within the A2 domain
Author(s) -
BOVENSCHEN N.,
VAN STEMPVOORT G.,
VOORBERG J.,
MERTENS K.,
MEIJER A. B.
Publication year - 2006
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1111/j.1538-7836.2006.01965.x
Subject(s) - immunoglobulin light chain , chemistry , ldl receptor , cleavage (geology) , thrombin , binding site , receptor , recombinant dna , endocytic cycle , plasma protein binding , heavy chain , microbiology and biotechnology , antibody , biophysics , lipoprotein , biochemistry , biology , cholesterol , gene , endocytosis , immunology , paleontology , platelet , fracture (geology)
Summary. Background: Low‐density lipoprotein receptor‐related protein (LRP) is an endocytic receptor that contributes to the clearance of coagulation factor (F) VIII from the circulation. Previously, we have demonstrated that region Glu 1811 ‐Lys 1818 within FVIII light chain constitutes an important binding region for this receptor. We have further found that FVIII light chain and intact FVIII are indistinguishable in their LRP‐binding affinities. In apparent contrast to these observations, a second LRP‐binding region has been identified within A2 domain region Arg 484 ‐Phe 509 of FVIII heavy chain. Objective: In this study, we addressed the relative contribution of FVIII heavy chain in binding LRP. Methods and Results: Surface plasmon resonance analysis unexpectedly showed that FVIII heavy chain poorly associated to the receptor. The binding to LRP was, however, markedly enhanced upon cleavage of the heavy chain by thrombin. The A2 domain, purified from thrombin‐activated FVIII, also showed efficient binding to LRP. Competition studies employing a recombinant antibody fragment demonstrated that region Arg 484 ‐Phe 509 mediates the enhanced LRP binding after thrombin cleavage. Conclusions: We propose that LRP binding of non‐activated FVIII is mediated via the FVIII light chain while in activated FVIII both the heavy and light chain contribute to LRP binding.