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Abstract
Author(s) -
O. Iqbal,
M. M. Sayeed
Publication year - 2003
Publication title -
journal of thrombosis and haemostasis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 178
eISSN - 1538-7836
pISSN - 1538-7933
DOI - 10.1111/j.1538-7836.2003.tb00113.x
Subject(s) - citation , library science , computer science , political science
P1315 Phosphorylation of platelet proteins in hemodialysed patients is different than in control donors NIA Exhibition Area 09:30 16th July, 2003 Session Type: Posters Subject area: Other platelets Session title: Platelets: clinical aspects II Abstract: P1315 Authors: B. Walkowiak* +, W. Tanski+ & W. Koziolkiewicz+ *Technical University of Lodz, Poland; +Medical University of Lodz, Poland It is commonly accepted that bleeding tendency in patients with renal failure results from a toxic effect of uremic plasma. However, hemodialysis significantly improve biochemical parameters of uremic blood, bleeding still is a major complication. Moreover, in vitro test for platelet aggregation indicates impaired platelet function in response to ADP. Results of our earlier studies allowed us to report that hemodialysis is able to normalise calcium ions homeostasis and mobilisation in blood platelets and also normalise platelet membranes fluidity. Platelet adhesion to immobilised collagen was also improved by hemodialysis. Our present research was devoted to finding out a possible difference in a pattern of tyrosine phosphorylation on proteins of blood platelet washed out from both pharmaceutically and hemodialysis treated patients and also from health donors. We have used radiolabelled monoclonal antibody PY20 for selective and specific detection of phosphotyrosine present on proteins immobilised at a PVDF membrane after electrotransfer from SDS-PAGE separation. For analysis were subjected samples of blood platelets in plasma just before and after aggregation process triggered with ADP. It was found that the pattern of phosphorylated platelet proteins was significantly different in hemodialysed patients when compared to both control and pharmaceutically treated patients. We conclude, that unlimited and uncontrolled contact of blood platelets with artificial surface of dialysing membrane can results in changes in signalling and thus in functioning of blood platelets. file:///E|/working/LAXMI-PRASAD/WileyML-3G/deepak/31-Jan/Wednesday/Abstract%20P1315.html [1/31/2014 3:51:00 PM]