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Detection of dengue virus in sera of Brazilian blood donors
Author(s) -
Dias Leny Lobo,
Amarilla Alberto Anastacio,
Poloni Telma Regina,
Covas Dimas Tadeu,
Aquino Victor Hugo,
Figueiredo Luiz Tadeu Moraes
Publication year - 2012
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2012.03729.x
Subject(s) - dengue fever , virology , dengue virus , outbreak , virus , asymptomatic , serotype , biology , genotype , polymerase chain reaction , medicine , gene , biochemistry
BACKGROUND: Dengue is the most important arboviral disease in the world. Dengue viruses (DENVs) have produced huge outbreaks in Brazil in the past 25 years with more than 5 million reported cases. During these epidemics, asymptomatic individuals infected with DENV could donate blood and serve as a source of virus dissemination in the community. Here, we studied the circulation of DENV in healthy individuals during an epidemic outbreak. STUDY DESIGN AND METHODS: The study included 500 serum samples from healthy blood donors collected at the Hemotherapy Center of Ribeirão Preto, Brazil, during a dengue outbreak. The presence of DENV RNA in the serum samples was screened by real‐time reverse transcription–polymerase chain reaction (PCR). The virus serotype was determined by a heminested PCR procedure. A partial fragment of the NS5 gene sequence was used for phylogenetic analysis. RESULTS: DENV RNA was detected in the serum sample of 2 of 500 (0.4%) individuals. Both of them were infected with DENV‐3 Genotype III, a virus that has been circulating in Brazil in the past decade. CONCLUSION: Individuals with asymptomatic DENV infection can be blood donors and serve as a source of virus dissemination in the community. Further studies are needed to determine the risk of recipient infection by DENV as a result of transfusion in Brazil, especially during epidemic periods.