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Adsorption of chain type–specific ABO antibodies on Sepharose‐linked A and B tetrasaccharides
Author(s) -
Lindberg Linda,
Theinert Katharina,
Liu Jining,
Holgersson Jan
Publication year - 2012
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2012.03706.x
Subject(s) - immunoadsorption , abo blood group system , chemistry , antibody , sepharose , blood type (non human) , tetrasaccharide , biochemistry , microbiology and biotechnology , immunology , enzyme , biology , polysaccharide
BACKGROUND: Antigen‐specific removal of anti‐A and anti‐B on immunoadsorption columns carrying the blood group A and B trisaccharides is one important component of some protocols used in ABO‐incompatible organ transplantation. Because ABO antibodies exist requiring parts of the core saccharide chain for binding, the anti‐A and ‐B–binding capacity of individual and combined, Sepharose‐linked Types 1 through 4 A and B tetrasaccharides with that of the A and B trisaccharides was compared. STUDY DESIGN AND METHODS: Sepharose‐linked A and B tri‐ and tetrasaccharides were used to adsorb anti‐A and ‐B from pooled blood group O serum. Remaining chain type–specific anti‐A and ‐B were detected and quantified in enzyme‐linked immunosorbent assays using wells coated with neoglycoproteins or recombinant mucins carrying A and B determinants on defined core saccharide chains. RESULTS: Significantly more anti‐A Type 3‐ and 4‐specific immunoglobulin (Ig)G remained after adsorption on the A trisaccharide and the A Type 1 and A Type 2 tetrasaccharide than after adsorption on the A Types 3 and 4 tetrasaccharides. Selective adsorption of chain type–specific IgG anti‐B was detected on Sepharose‐linked B tetrasaccharides. In contrast, there were no chain type–specific IgM anti‐A or ‐B. A combination of the A or B tetrasaccharides adsorbed a larger fraction of the IgG anti‐A and ‐B repertoires than the corresponding trisaccharides. CONCLUSION: There are chain type–specific anti‐A and anti‐B IgG, and an adsorber based on a combination of Types 1 through 4 A or B tetrasaccharides will be a more efficient adsorber than an adsorber based on the A or B trisaccharides.