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Characteristics of thawed autologous umbilical cord blood
Author(s) -
Rosenau Emma H.,
Sugrue Michele W.,
Haller Michael,
Fisk Diann,
Kelly Susan S.,
Chang Myron,
Hou Wei,
Eldjerou Lamis,
Slayton William,
Cogle Christopher R.,
Wingard John R.
Publication year - 2012
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2011.03556.x
Subject(s) - umbilical cord , cryopreservation , cord blood , medicine , andrology , biology , surgery , immunology , embryo , microbiology and biotechnology
BACKGROUND: Autologous umbilical cord blood (AutoUCB) has historically been cryopreserved for potential use in hematopoietic transplantation. Increasingly, private AutoUCB banking is performed for therapies unavailable today. A Phase I trial using AutoUCB treatment for early pediatric Type 1 diabetes afforded us an opportunity to analyze characteristics of AutoUCBs. STUDY DESIGN AND METHODS: Twenty AutoUCBs from AABB‐accredited private cord blood banks (CBBs) were evaluated for collection, processing, cryopreservation, and thaw characteristics. Using a standardized thaw‐wash method, AutoUCBs were assessed for viable total nucleated cells (vTNCs), viable CD34+ (vCD34+), and colony‐forming unit–granulocyte‐macrophage counts. Postthaw %vTNC recoveries were compared against processing characteristics and analyzed according to processing method, cryopreservation volume, concentration, container, and length of storage. RESULTS: AutoUCB collection volumes (19.9‐170 mL), cryopreserved TNC counts (7.6 × 10 7 ‐3.34 × 10 9 ), %TNC processing recoveries (39%‐100%), postthaw %vTNC recoveries (58%‐100%), and %vCD34+ recoveries (26%‐96%) varied widely. Regarding cell dose requirements, only 11% of evaluable AutoUCBs achieved the minimum TNC count of at least 9.0 × 10 8 to meet the National Cord Blood Inventory banking threshold, and only 50% met the minimum of 5.0 × 10 8 TNC count for Food and Drug Administration cord blood licensure eligibility. %vTNC recoveries correlated with %vCD34+ recoveries (R = 0.7; p = 0.03). Length of storage, cryopreservation volume, concentration, and container type did not affect postthaw %vTNC recoveries. CBB processing method, however, was associated with %vTNC postprocessing recoveries, with unmanipulated and plasma‐depleted AutoUCBs having the highest postthaw %vTNC recovery, followed by RBC‐depleted and density gradient–separated AutoUCBs. CONCLUSION: The high variability and low counts found in AutoUCB banking suggest that further standardization of characterization, collection, and processing procedures is needed.