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Volume‐reduced platelet concentrates: optimization of production and storage conditions
Author(s) -
van der Meer Pieter F.,
Bontekoe Ido J.,
Kruit Guido,
Peeters Geert,
van Toledo Pleun J.,
Tomson Bert,
de Korte Dirk
Publication year - 2012
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2011.03357.x
Subject(s) - volume (thermodynamics) , production (economics) , blood preservation , platelet , medicine , andrology , economics , physics , thermodynamics , macroeconomics
BACKGROUND: Plasma can be removed from platelet (PLT) concentrates (PCs) when volume reduction for PLT transfusion is indicated. Volume‐reduced PCs are currently produced from pooled buffy coat (BC) PCs or apheresis PCs by pretransfusion volume reduction, followed by transfer to a syringe for immediate transfusion. We evaluated the maximal storage time of the volume‐reduced PCs in gas‐permeable containers. STUDY DESIGN AND METHODS: Volume‐reduced PCs were produced from BC‐derived and apheresis PCs by hard‐spin centrifugation. Supernatant was removed and the PLTs were resuspended in 20 mL of retained original PC and had PLT concentrations ranging from 10.8 × 10 9 to 13.8 × 10 9 PLTs/mL. Volume‐reduced PCs were stored either in syringes or in containers made from diethylhexyl phthalate (DEHP)‐polyvinylchloride (PVC) or butyryl trihexyl citrate (BTHC)‐PVC plastic. Units were sampled at t = 0, 1, 3, and 6 hours for in vitro measurements. RESULTS: When prepared from 2‐day‐old PCs (n = 4), volume‐reduced PCs from BCs in a syringe had a pH 37°C of 5.76 ± 0.04 at t = 6 hours after volume reduction. In the DEHP‐PVC container, pH was 5.85 ± 0.15 (not significant), and in the BTHC‐PVC, 6.34 ± 0.16 (p < 0.001), at t = 6 hours. When made from 7‐day‐old PCs, pH was lower for all storage conditions: 5.68 ± 0.06 in the syringe, 5.70 ± 0.09 in the DEHP‐PVC container (not significant), and 6.07 ± 0.24 in the BTHC‐PVC container (p < 0.01) at t = 6 hours. Volume‐reduced 2‐day‐old apheresis PCs had a pH of 6.47 ± 0.20 at t = 6 hours. CONCLUSIONS: Adult‐dose PCs derived from BC or apheresis can be volume‐reduced to approximately 20 mL in a closed gas‐permeable system. Volume‐reduced PCs in BTHC‐PVC containers retain a mean pH of more than 6.0 up to 6 hours after production. Syringes allow only 3 hours of storage.