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FCGR3B * 03 allele inheritance pattern in Brazilian families and some new variants of gene FCGR3B
Author(s) -
Terzian Claudia C.N.,
Chiba Akemi K.,
Santos Viviani C.,
Silva Neusa P.,
Bordin Jose O.
Publication year - 2012
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2011.03326.x
Subject(s) - genetics , biology , allele , gene
BACKGROUND: The FCGR3B gene encoding the FcyRIIIb receptor for immunoglobulin G has three polymorphic forms known as HNA‐1a, HNA‐1b, and HNA‐1c, encoded by the alleles FCGR3B * 01 , FCGR3B * 02 , and FCGR3B * 03 , respectively. It is not clear whether the inheritance of the FCGR3B * 03 allele, which encodes the HNA‐1c, is linked or not to the other two alleles. The objective of this study was to identify the inheritance pattern of the FCGR3B * 03 allele in Brazilians. STUDY DESIGN AND METHODS: Blood samples from nine families with at least one FCGR3B * 03 (+) member, totalizing 47 individuals, were studied. The presence of the FCGR3B * 01 , FCGR3B * 02 , and FCGR3B * 03 alleles was detected by the polymerase chain reaction with sequence‐specific priming method, and all DNA samples were sequenced. RESULTS: In three of the nine studied families, the FCGR3B * 03 was passed down with the FCGR3B * 02 , while in one family the FCGR3B * 03 was inherited in linkage with FCGR3B * 01 . The other families were not informative regarding FCGR3B * 03 inheritance. Sequencing showed for the first time one single‐nucleotide polymorphism at Position 264 resulting from a simple substitution C→T; three other different substitutions at Position 230, T→A, T→G; and the presence of three nucleotides at Position 230 (T, G, and A). The previously reported variants FCGR3B * 01A227G and FCGR3BG330T were also found. CONCLUSION: In this Brazilian FCGR3B * 03 (+) group we found that the inheritance of FCGR3B * 03 took place by a linkage to FCGR3B * 02 or to FCGR3B * 01 . Linkage of FCGR3B * 03 to FCGR3B * 02 was the most common. Additionally, we report SNPs that have not been described, suggesting that they might be more common than previously thought.