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Flow cytometry evaluation of red blood cells mimicking naturally occurring ABO subgroups after modification with variable amounts of function‐spacer‐lipid A and B constructs
Author(s) -
Hult Annika K.,
Frame Tom,
Chesla Scott,
Henry Stephen,
Olsson Martin L.
Publication year - 2012
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2011.03268.x
Subject(s) - abo blood group system , flow cytometry , serology , antigen , serial dilution , microbiology and biotechnology , cytometry , biology , immunology , chemistry , medicine , pathology , antibody , alternative medicine
BACKGROUND: Kodecytes bearing synthetic blood group A and B antigens are increasingly being used in transfusion laboratories as serologic mimics of red blood cell (RBC) A weak and B weak subtypes. The aim of this study was to compare the flow cytometry profile of kodecytes with native ABO subgroups. STUDY DESIGN AND METHODS: A series of A/B kodecytes, each with decreasing A or B antigen expression, were prepared from group O RBCs that were modified with dilutions of function‐spacer‐lipid KODE technology (FSL) constructs representing a wide serologic range. Using an established flow cytometry method designed for the detection of low levels of A/B antigens, kodecyte profiles were compared with those of native subgroup cells. RESULTS: Kodecytes with positive tube serology from 4+ to 1+ were created with 15 to 2 µg/mL FSL‐A or 78 to 10 µg/mL FSL‐B transformation solutions. The kodecytes created with higher concentrations of FSL constructs revealed a uniform and/or even distribution of antigens as seen by a single flow cytometry peak more narrow than the broader peaks produced with lower FSL concentrations similar to those found in native A x and most B weak subgroups. CONCLUSIONS: Although kodecytes are created artificially, they can be designed to mimic the serologic and flow cytometric profiles of native ABO subgroup RBCs.