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Validation of a microbial detection system for use with ACD‐A platelets with PAS III platelet additive solution
Author(s) -
Dumont Larry J.,
Hay Shau.,
Herschel Louise,
Brantigan Barbara,
Houghton Jaime,
Elfath M. Dean,
Brecher Mark E.
Publication year - 2011
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2011.03149.x
Subject(s) - anaerobic exercise , platelet , bottle , colony forming unit , microbiology and biotechnology , agar plate , plateletpheresis , chemistry , apheresis , andrology , medicine , biology , bacteria , immunology , materials science , physiology , genetics , composite material
BACKGROUND: The BacT/ALERT microbial detection system (BTA) is used for testing leukoreduced apheresis platelets (LR‐AP) in plasma. Platelet additive solutions (PASs) such as InterSol (PAS III) may be used to reduce the amount of plasma transfused in LR‐AP. This study evaluated the performance of the two‐bottle BTA testing scheme in the recovery of seeded microorganisms from LR‐AP in InterSol‐plasma compared to a reference plate culture method. STUDY DESIGN AND METHODS: Hyperconcentrated, double LR‐AP were collected from healthy donors; InterSol was added (65% Intersol:35% plasma), equally divided into two containers, and then inoculated with an isolate of 1 of 10 clinically relevant index organisms at two levels. Aerobic (BPA) and anaerobic (BPN) BTA bottles were inoculated with 4 mL each of the inoculated LR‐AP, and blood agar plates (BAPs) for aerobic and anaerobic culture (0.5 mL each). RESULTS: Zero false‐positives from 103 bottle pairs were observed. All 400 two‐bottle BTA tests were positive within 24 hours, except for Propionibacterium acnes (maximum time‐to‐detection of 86.4 hr) and 13 of 20 pairs of Streptococcus viridans (maximum time‐to‐detection of 31.7 hr). Thirteen of 400 BAP two‐plate tests were negative for starting bacterial concentrations of 10 colony‐forming units (CFUs)/mL or less. At 40 CFUs/mL or less, BTA was 100% positive while BAP was 94% positive. CONCLUSION: Seeded organism recovery was superior in the two‐bottle BTA test system compared to the two‐plate BAP system using InterSol platelets (PLTs). This performance is comparable to previously published results for PLTs in plasma. The use of InterSol does not appear to have a detrimental effect on the performance of the two‐bottle BTA system.