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Evaluation of overnight hold of whole blood at room temperature before component processing: effect of red blood cell (RBC) additive solutions on in vitro RBC measures
Author(s) -
van der Meer Pieter F.,
Cancelas Jose A.,
Cardigan Rebecca,
Devine Dana V.,
Gulliksson Hans,
Sparrow Rosemary L.,
Vassallo Ralph R.,
de WildtEggen Janny,
BaumannBaretti Bärbel,
Hess John R.
Publication year - 2011
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2010.02959.x
Subject(s) - hemolysis , buffy coat , degree celsius , red blood cell , mannitol , chemistry , whole blood , saline , hemoglobin , red cell , andrology , guanosine , platelet , medicine , biochemistry , anesthesia , surgery , physics , thermodynamics
BACKGROUND: Whole blood (WB) can be held at room temperature (18‐25°C) up to 8 hours after collection; thereafter the unit must be refrigerated, rendering it unsuitable for platelet (PLT) production. Overnight hold at room temperature before processing has logistic advantages, and we evaluated this process in an international multicenter study for both buffy coat (BC)‐ and PLT‐rich plasma (PRP)‐based blood components and compared three red blood cell (RBC) additive solutions (ASs) for their ability to offset effects of overnight hold. STUDY DESIGN AND METHODS: Nine centers participated; seven used the BC method, and two used the PRP method. Four WB units were pooled and split; 1 unit was processed less than 8 hours from collection (Group A), and the other three (Groups B, C, and D) were held at room temperature and processed after 24 to 26 hours. RBCs in Groups A and B were resuspended in saline‐adenine‐glucose‐mannitol, Group C in phosphate‐adenine‐guanosine‐glucose‐saline‐mannitol, and Group D in ErythroSol‐4 RBCs were stored at 2 to 6°C for 49 days. RESULTS: RBCs from overnight‐held WB had lower 2,3‐diphosphoglycerate (2,3‐DPG) and higher adenosine triphosphate (ATP). At the end of storage there were no differences between groups, apart from a slightly higher hemolysis in Group B. ErythroSol‐4 showed a slightly higher initial ATP and 2,3‐DPG content, but at the end of storage no differences were found. CONCLUSION: Overnight hold of WB before processing has no lasting deleterious effects on in vitro quality of subsequently prepared components. The use of different RBC ASs did not appear to offer significant advantages in terms of RBC quality at the end, regardless of the processing method.