Premium
RHD positive among C/E+ and D− blood donors in Denmark
Author(s) -
Christiansen Mette,
Sørensen Betina S.,
Grunnet Niels
Publication year - 2010
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2010.02611.x
Subject(s) - genotyping , serology , exon , genotype , polymerase chain reaction , rh blood group system , population , mutation , subtyping , microbiology and biotechnology , blood type (non human) , serotype , virology , genomic dna , biology , gene , medicine , genetics , immunology , antibody , blood typing , programming language , environmental health , computer science
BACKGROUND: Many different partial and weak D types have been reported, and most of these are easily detected by serology. However, 17 Del types have also been described, with a very low expression of the D antigen, only detectable by absorption‐elution techniques, and these may elicit the development of an anti‐D. A genomic test of C/E+ and D− blood donors was initiated, to be able to categorize them correctly as D+ or −. STUDY DESIGN AND METHODS: We analyzed all C/E+ and D− donors within our donor population of 22,000 donors with an initial test for RHD Exon 10. In case of a positive reaction, the genotype was further analyzed by sequence‐specific polymerase chain reaction or nucleotide sequencing of the RHD gene. CONCLUSIONS: Of 233 donors analyzed, seven were found positive for RHD Exon 10, and four of these were Del, corresponding to 1.7%. We report here a new mutation in the RHD gene. A correct assignment of all blood donors as D+ or D− is not possible using serotyping alone; genotyping offers the only exact categorization of all cases.