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Application of the bactericidal activity of ε‐poly‐ l ‐lysine to the storage of human platelet concentrates
Author(s) -
Tanaka Shigenori,
Hayashi Tomoya,
Tateyama Hidemi,
Matsumura Kazuaki,
Hyon SuongHyu,
Hirayama Fumiya
Publication year - 2010
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2009.02503.x
Subject(s) - chemistry , platelet , bacillus cereus , lactate dehydrogenase , bacteria , food science , microbiology and biotechnology , biochemistry , biology , enzyme , immunology , genetics
BACKGROUND: ε‐Poly‐ l ‐lysine (ε‐PLL) is a polypeptide comprising approximately 30 l ‐lysine subunits generated by bond formation between α‐carboxy and ε‐amino groups. It is an approved antimicrobial food preservative in Japan. However, the efficacy of ε‐PLL as an antibacterial additive for storage of human platelet concentrates (PCs) is not known. STUDY DESIGN AND METHODS: Staphylococcus aureus , Bacillus cereus, and Klebsiella oxytoca (20 colony‐forming units/mL) were inoculated into 100% plasma PCs or PCs containing 80% platelet (PLT) additive solution with 5.0 mmol/L potassium and 1.5 mmol/L magnesium (PAS‐IIIM) and 20% plasma (PAS‐IIIM PCs). Next, a range of ε‐PLL concentrations up to 200 and 50 µg/mL were added to plasma PCs and PAS‐IIIM PCs, respectively, and the bacterial count was determined on Days 1, 2, 5, and 8. The quality of the PCs was also determined. RESULTS: Bacterial growth was inhibited at ε‐PLL concentrations of 200 and 50 µg/mL in the plasma and PAS‐IIIM PCs after 8 days of incubation. The percentage of CD62P‐positive PLTs was higher in plasma PCs treated with 200 µg/mL ε‐PLL and in PAS‐IIIM PCs treated with 50 µg/mL ε‐PLL than in the respective controls without ε‐PLL. There were no remarkable differences in the other variables, that is, PLT number, mean PLT volume, pH, aggregability, percentage of PAC‐1–positive cells, lactate dehydrogenase release, and plasma K and Na concentrations between the ε‐PLL–treated PCs and the controls. CONCLUSIONS: ε‐PLL inhibited the growth of bacteria in the PCs and did not considerably affect the quality of PCs, except CD62P expression. Further studies are required to estimate the in vivo effectiveness and safety of ε‐PLL–treated PCs.