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Anti‐Hr B and anti‐hr B revisited
Author(s) -
Pham BachNga,
Peyrard Thierry,
Tourret Séverine,
Beolet Marylise,
Many Hélène,
Juszczak Geneviève,
Roussel Michèle,
KapplerGratias Sandrine,
Rouger Philippe,
Le Pennec PierreYves
Publication year - 2009
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2009.02307.x
Subject(s) - antigen , antibody , loss of heterozygosity , serology , haplotype , immune system , biology , phenotype , genetics , immunology , gene , allele , microbiology and biotechnology
BACKGROUND: Since their description in the 1970s, anti‐Hr B (antibody against a high‐prevalence Rh antigen) and anti‐hr B (anti‐e–like antibody) are still a subject of debate about representing two aspects of a global immune response or being two independent antibodies. STUDY DESIGN AND METHODS: The aim of this study was to evaluate the immune response against the antigens of Rh system of 30 individuals presenting a hr B (RH31)− phenotype. Genomic analysis of RH genes was performed in all individuals. RESULTS: Among the 30 individuals, 27 had a Hr B (RH34)− phenotype. No immunization against Rh antigens was found in 16 individuals. Three individuals made anti‐D only, whereas six individuals made anti‐Hr B (four with anti‐hr B and two without anti‐hr B ) and two individuals made anti‐hr B without anti‐Hr B . Among the 30 individuals, three had a Hr B + phenotype. No immunization against Rh antigens was found in one individual, whereas two individuals made anti‐hr B ; the genomic analysis of selected individuals showed the presence of a (C)ce s haplotype, either Type 1 or Type 2, and a DIII Type 5 ce s haplotype, in the homozygous state, in compound heterozygosity with each other or in heterozygosity with a DcE haplotype. Genomic data were in accordance with serologic data. CONCLUSION: Our data provide the evidence that anti‐Hr B and anti‐hr B are independent antibodies, defining two different specificities. These antibodies may be produced by individuals expressing variants of RhCE protein. Serologic and molecular data indicate that e antigen encoded by the (C)ce s haplotype is a partial antigen. In individuals carrying a (C)ce s haplotype, the risk and the type of alloimmunization to Rh antigens are related to the second Rh haplotype.