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Immunophenotypical, morphologic, and functional characterization of maturation‐associated plasmacytoid dendritic cell subsets in normal adult human bone marrow
Author(s) -
MartínMartín Lourdes,
Almeida Julia,
HernándezCampo Pilar María,
Sánchez María Luz,
Lécrevisse Quentin,
Orfao Alberto
Publication year - 2009
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2009.02170.x
Subject(s) - plasmacytoid dendritic cell , cd34 , biology , bone marrow , myeloid , immunology , interleukin 3 receptor , dendritic cell , antigen , stem cell , microbiology and biotechnology
BACKGROUND: Information about maturation of plasmacytoid dendritic cell precursors (pre‐pDCs) in normal bone marrow (BM) remains limited. STUDY DESIGN AND METHODS: Immunophenotypical, morphologic, and functional changes associated with maturation of pre‐pDCs were analyzed in adult normal human BM (n = 45). RESULTS: Three pre‐pDC maturation stages, with an increasingly higher degree of maturity, were systematically identified: CD34++/HLA‐DR++/+++/CD123++/CD45+/++ (Stage I), CD34+/HLA‐DR+/++/CD123++/+++/CD45+/++ (Stage II), and CD34−/HLA‐DR++/CD123++/+++/CD45++ (Stage III) cells. Lymphoid‐ and early myeloid‐associated molecules, as well as CD86, were coexpressed in Stage I pre‐pDCs, being down regulated afterward. CD304 and CD85j appeared in Stage I, progressively increasing their levels thereafter. Interestingly, cutaneous lymphocyte‐associated antigen was heterogeneously expressed throughout the maturation, particularly in Stage I pre‐pDCs. The morphologic appearance of Stage I pre‐pDCs was consistent with their undifferentiated stage, while Stage II/III cells showed morphologic features of more differentiated cells. From the functional point of view, only Stage II and Stage III pre‐pDCs were capable of inducing allogeneic T‐cell proliferation, the later subset also showing interferon‐γ secretion; in contrast, Stage I pre‐pDCs showed the highest endocytic ability. CONCLUSION: In summary, three maturation stages of pre‐pDCs can be identified in adult normal BM, which show unique phenotypical, morphologic, and functional characteristics; these results provide a frame of reference for a better understanding of pDC malignancies.