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Maintenance of platelet in vitro properties during 7‐day storage in M‐sol with a 30‐hour interruption of agitation
Author(s) -
Wagner Stephen J.,
Myrup Andrew,
Awatefe Helen,
ThompsonMontgomery Dedeene,
Hirayama Junichi,
Skripchenko Andrey
Publication year - 2008
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2008.01900.x
Subject(s) - chemistry , bicarbonate , platelet , chromatography , plateletpheresis , andrology , medicine , apheresis , organic chemistry
BACKGROUND: Extensive periods without agitation can occasionally occur during platelet (PLT) shipment and can affect PLT quality during 5‐ to 7‐day storage. The use of buffer‐containing PLT additive solutions (ASs) may better preserve PLT quality during storage by maintaining PLT pH and other in vitro variables. A newly described bicarbonate‐containing AS, M‐sol, was compared to plasma for preservation of whole blood–derived PLT concentrates in which a 30‐hour interruption of agitation was included. STUDY DESIGN AND METHODS: ABO‐identical PLT‐rich plasma intermediate products were pooled in sets of four, split, and centrifuged with subsequent plasma expression (n = 12). Two units were resuspended with M‐sol AS to produce a 70 percent solution/30 percent plasma PLT concentrate; 2 units were resuspended in 100 percent plasma. One M‐sol resuspended unit and 1 plasma unit were held on a laboratory bench in a standard shipping box for 30 hours between Day 2 and Day 3, while the other M‐sol and plasma unit were continuously agitated. Standard in vitro testing for PLT quality variables on each set of 4 units was performed during storage (n = 12). RESULTS: Interrupting agitation of PLTs suspended in M‐sol resulted in less of a pH decrement during storage than that of PLTs suspended in 100 percent plasma. On Days 5 and 7, the pH differences between M‐sol and plasma units were 0.56 and 0.75 pH units, respectively (p < 0.0003). In addition, PLTs suspended in M‐sol and subjected to an interruption of agitation had lesser Day 7 CD62+ cells, glucose utilization, and lactate production and greater hypotonic stress response, morphology, swirling, and aggregation response than those suspended in plasma (p ≤ 0.005). CONCLUSION: The in vitro properties of PLTs suspended in 70 percent M‐sol/30 percent plasma and subjected to a 30‐hour interruption of agitation are better maintained during 7‐day storage than those of matched units suspended in plasma.