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Effects of Mirasol PRT treatment on storage lesion development in plasma‐stored apheresis‐derived platelets compared to untreated and irradiated units
Author(s) -
Picker Susanne M.,
Steisel Alexander,
Gathof Birgit S.
Publication year - 2008
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2008.01778.x
Subject(s) - apheresis , platelet , lactate dehydrogenase , lesion , andrology , plateletpheresis , chemistry , medicine , biochemistry , surgery , enzyme
BACKGROUND: The aim of this study was to examine the effects of a new riboflavin‐based pathogen reduction technology (PRT), the Mirasol PRT process (Navigant Biotechnologies) on platelet (PLT) storage lesion development. STUDY DESIGN AND METHODS: A three‐arm in vitro study was conducted comparing cell quality of apheresis PLTs (n = 12 each) treated with Mirasol PRT (M) to untreated (C) and gamma‐irradiated units (X) collected from the same donors and stored for up to 7 days under equal conditions. RESULTS: PLT count, lactate dehydrogenase, and K + release of M units were not significantly different from C units, indicating retention of cell integrity during storage. The immediate effect (Day 1) of PRT treatment was a significant decrease in hypotonic shock response (M, 80.6 ± 7.8% vs. 89.2 ± 8.3%) and aggregation (M, 85.7 ± 15.2%/min vs. 111.8 ± 31.5%/min) as well as a significant acceleration of mitochondrial membrane depolarization (M, 1.43 ± 0.44% vs. 0.91 ± 0.27%) and P‐selectin expression (M, 38.4 ± 13.8% vs. 15.8 ± 7.7%) resulting in lower swirl scores on Day 5 (1.5 ± 0.7 vs. 2.7 ± 0.4). Significantly higher glucose consumption (60 ± 13 nmol/10 12 cells/hr vs. 31 ± 9 nmol/10 12 cells/hr) and lactate production rates (82 ± 17 nmol/10 12 cells/hr vs. 40 ± 8 nmol/10 12 cells/hr) caused higher acidity in treated units (pH on Day 5, 6.97 ± 0.15 vs. 7.42 ± 0.10). After PRT treatment, oxidative metabolism was still active and, from calculation of oxygen consumption (1.09 ± 0.23 nmol/min/10 9 PLTs), appeared to be up regulated relative to controls (0.76 ± 0.27 nmol/min/10 9 PLTs). CONCLUSION: Although storage variables clearly showed the effects of PRT treatment, apheresis PLTs treated with Mirasol PRT retained cell quality during 5 days of storage without loss of mitochondria‐based oxidative respiration.