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Genetic variation of FUT2 in Ovambos, Turks, and Mongolians
Author(s) -
Soejima Mikiko,
Nakajima Tamiko,
Fujihara Junko,
Takeshita Haruo,
Koda Yoshiro
Publication year - 2008
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2008.01710.x
Subject(s) - single nucleotide polymorphism , haplotype , genetics , biology , locus (genetics) , allele , population , genotype , genetic variation , coding region , gene , medicine , environmental health
BACKGROUND: Many single‐nucleotide polymorphisms (SNPs) have been identified in the coding region of the FUT2 locus, which encodes secretor type α(1,2)fucosyltransferase. These SNPs are highly population‐specific. STUDY DESIGN AND METHODS: The 1121‐bp polymerase chain reaction (PCR) product containing the whole FUT2 coding region in three human populations, Ovambos (n = 74), Turks (n = 70), and Mongolians (n = 118), was sequenced. The haplotypes consisting of novel SNPs were determined by sequencing cloned inserts, and the haplotypes consisting of already reported SNPs were inferred by free computer software (PHASE). The functional significance of novel SNPs by transient expression study was also examined. RESULTS: Twenty‐four SNPs were found including seven novel SNPs (i.e., 4G > A, 244G > A, 442C > A, 489G > A, 569G > A, 665G > A, and 950C > T). A transient expression study suggested that the 244G > A, 569G > A, and 950C > T SNPs are enzyme‐inactivating mutations. CONCLUSION: This study identified 24 SNPs in the FUT2 gene, of which 7 were novel. The frequencies of alleles and genotypes were determined in Ovambos, Turks, and Mongolians. The allelic composition of each population was similar to those of geographically closer populations.