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The influence of simulated shipping conditions (24‐ or 30‐hr interruption of agitation) on the in vitro properties of apheresis platelets during 7‐day storage
Author(s) -
Wagner Stephen J.,
Vassallo Ralph,
Skripchenko Andrey,
Einarson Mindy,
Seetharaman Shalini,
Moroff Gary
Publication year - 2008
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2008.01690.x
Subject(s) - apheresis , platelet , plateletpheresis , blood product , chemistry , medicine , zoology , surgery , biology
BACKGROUND: Platelet (PLT) components undergo interruption of agitation during shipment. Studies have demonstrated maintenance of PLT quality of whole blood–derived PLT concentrates during a 24‐hour interruption of agitation, but data are not available for apheresis PLTs in 100 percent plasma. STUDY DESIGN AND METHODS: Apheresis PLTs were collected with one of two commercially available separators (Amicus, Fenwal, Inc.; or Trima Accel, Gambro BCT) to provide two identical PLT products, each with approximately 3 × 10 11 to 4.5 × 10 11 PLTs. The control product was continuously agitated. The test product was subjected to a continuous 24‐ or 30‐hour period of interrupted agitation between Day 2 and Day 3 of storage by placement in a standard shipping box at room temperature. RESULTS: Interrupting agitation for 24 or 30 hours influenced in vitro PLT properties to various degrees. After 5 days of storage, pH levels were judged to be well maintained after 24 hours without agitation for PLTs collected with both separators (pH < 6.2, Trima [0/12] and Amicus [0/12]). The changes in other variables associated with the retention of postinfusion viability were also considered limited and acceptable in units subjected to a 24‐hour interruption of agitation. After 7 days of storage including a 24‐hour interruption of agitation, Trima PLTs better maintained PLT properties compared to Amicus PLTs. With a 30‐hour period, both Trima and Amicus PLTs were deemed satisfactory for pH at 5 days, but not at 7 days (pH < 6.2: Day 5, Trima [0/23] and Amicus [1/22]; Day 7, Trima [5/23] and Amicus [4/17]). CONCLUSION: Based on the retention of pH levels of at least 6.2, apheresis PLT quality was maintained for 5 days with a 24‐hour and a 30‐hour interruption of agitation.