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Rapid detection of anti‐Lu b with recombinant Lu b protein and the particle gel immunoassay
Author(s) -
Seltsam Axel,
Agaylan Ashraf,
Grueger Daniela,
Meyer Oliver,
Blasczyk Rainer,
Salama Abdulgabar
Publication year - 2008
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2007.01596.x
Subject(s) - recombinant dna , immunoassay , streptavidin , antibody , microbiology and biotechnology , chemistry , chromatography , biotin , biology , immunology , biochemistry , gene
BACKGROUND: Until now, it was not possible to identify antibodies to red blood cells (RBCs) except with pretyped RBCs. Here, a novel method with particles coated with recombinant Lu b protein for detection of anti‐Lu b is described. STUDY DESIGN AND METHODS: Prokaryotic recombinant Lu b proteins were generated and coupled onto superparamagnetic particles coated with streptavidin. The coated particles were tested in the presence of different serum and plasma samples (13 anti‐Lu b , 6 anti‐Lu a , 20 other antibodies, and 35 serum samples from blood donors) with the particle gel immunoassay (ID‐PaGIA). RESULTS: Lu b ‐coated particles reacted with all 13 samples containing anti‐Lu b , but not with any samples lacking anti‐Lu b . In addition, the anti‐Lu b titers were higher with Lu b ‐coated particles than with Lu(a–b+) RBCs in almost all cases. CONCLUSION: Recombinant blood group proteins may be able to dispense with the need for RBCs for identification of certain RBC alloantibodies.