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Platelet function under high‐shear conditions from platelet concentrates
Author(s) -
JilmaStohlawetz Petra,
Horvath Michaela,
Eichelberger Beate,
Koren Daniela,
Jilma Bernd,
Panzer Simon
Publication year - 2008
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2007.01490.x
Subject(s) - platelet , medicine , shear (geology) , materials science , composite material
BACKGROUND: Platelet (PLT) collection and storage affect the functional capacity of PLTs in PLT concentrates (PCs). Therefore, PLTs' functional quality should be studied before transfusion. STUDY DESIGN AND METHODS: PCs (n = 15) were collected by a standard apheresis procedure (Trima, Gambro BCT) and were stored for 7 days. Samples were taken to assess PLT adhesion and aggregate formation by a cone and plate analyzer (Impact‐R, DiaMed) on Days 1, 3, 5, and 7 after harvesting. This device allows testing PLT function under high‐shear stress close to physiologic conditions. Concomitantly, P‐selectin expression and the residual responsiveness to TRAP‐6 were determined by flow cytometry. RESULTS: PLT adhesion, as measured by surface coverage, decreased during the entire observation period; likewise, the size of aggregates was significantly lower on Days 5 and 7 compared to Day 1 (p < 0.02). P‐selectin expression increased from Day 5 to Day 7 (p < 0.04), whereas TRAP‐6–inducible expression remained stable until Day 5 of storage and decreased significantly on Day 7 (p = 0.04). CONCLUSIONS: Our results show that high‐shear–induced PLT adhesion and aggregation on the polystyrene surface deteriorate upon storage, suggesting decreased PLT function in vivo. Thus, the Impact‐R may be a useful tool to assess the functional capacity of PLTs under various PLT harvesting and storage procedures.