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Microcalorimetry: a novel method for detection of microbial contamination in platelet products
Author(s) -
Trampuz Andrej,
Salzmann Simone,
Antheaume Jeanne,
Daniels Alma U.
Publication year - 2007
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2007.01336.x
Subject(s) - isothermal microcalorimetry , staphylococcus aureus , candida albicans , staphylococcus epidermidis , microbiology and biotechnology , escherichia coli , chemistry , microorganism , food science , contamination , colony forming unit , biology , bacteria , biochemistry , enthalpy , ecology , physics , genetics , quantum mechanics , gene
BACKGROUND: Measuring heat from replicating microorganisms in culture may be a rapid, accurate, and simple screening method for platelets (PLTs). Microcalorimetry for detection of microorganisms in in vitro contaminated PLT products was evaluated. STUDY DESIGN AND METHODS: Staphylococcus epidermidis , Staphylococcus aureus , Streptococcus sanguinis , Escherichia coli , Propionibacterium acnes, and Candida albicans were inoculated in single‐donor apheresis PLTs to achieve target concentrations of 10 5 , 10 3 , 10, or 1 colony‐forming units (CFU) per mL of PLTs. Contaminated PLTs in growth medium were incubated at 37°C for 5 days in a calorimeter. Positivity was defined as heat flow of at least 10 µW above the lowest value of the power–time curve. RESULTS: With microcalorimetry, inocula of 10 CFUs per mL PLTs could be detected with the following detection times: S. epidermidis (31.65 hr), S. aureus (24.24 hr), S. sanguinis (7.82 hr), E. coli (7.53 hr), P. acnes (73.57 hr), and C. albicans (43.77 hr). The detection time was less than 4 hr at 10 5 CFUs per mL PLTs for S. aureus , S. sanguinis , and E. coli . Noncontaminated PLTs remained negative. The total heat ranged from 2.8 ( S. sanguinis ) to 8.3 J ( E. coli ).The shape of the power–time curve was species‐specific and independent from the initial concentration of microorganisms. CONCLUSION: The detection limit of microcalorimetry was 1 to 10 CFUs per mL PLTs. Microcalorimetry is a promising novel method for detection of contaminated PLTs. Applying this method to all PLT products could reduce the frequency of transfusion‐related sepsis and prolong the shelf life of PLTs.