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Molecular genetic blood group typing by the use of PCR‐SSP technique
Author(s) -
Prager Martina
Publication year - 2007
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2007.01311.x
Subject(s) - genotyping , typing , serology , abo blood group system , polymerase chain reaction , allele , biology , genetics , medicine , genotype , antibody , gene
BACKGROUND: DNA‐based methods are useful for enhancing immunohematology typings. Ready‐to‐use Conformité Européenne (CE)‐marked test kits based on polymerase chain reaction with sequence‐specific priming (PCR‐SSP) have been developed, which enable the examination of weak, unexpected, or unclear serologic findings. DEVELOPMENT AND VALIDATION: Primers were designed according to established mutation databases. Proficiency testing for CE marking was performed in accordance with Directive 98/79EC of the European Parliament and of the Council of October 27, 1998 on in vitro diagnostic medical devices using pretyped in‐house and external samples. INTENDED USE: BAGene PCR‐SSP kits are in vitro diagnostic devices. Genotyping of ABO and RHD/RHCE as well as HPA and KEL , JK , and FY specificities has to be performed after the conclusion of the serologic determination. APPLICATION: Ready‐to‐use PCR‐SSP typing kits allow the determination of common, rare, or weak alleles of the ABO blood group, Rhesus, and Kell/Kidd/Duffy systems as well as alleles of the human platelet antigens. RESULTS: The investigations showed clear‐cut results in accordance with serology or molecular genetic pretyping. CONCLUSION: PCR‐SSP is a helpful supplementary technique for resolving most of the common problems caused by discrepant or doubtful serologic results, and it is an easy‐to‐handle robust method. Questionable cases in donor, recipient, and patient typing can be examined with acceptable cost.