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HPA‐13bw neonatal alloimmune thrombocytopenia and low frequency alloantigens: case report and review of the literature
Author(s) -
Bertrand Gerald,
Bianchi Frederic,
Alexandre Marie,
Quesne Jeannine,
Chenet Christophe,
Martageix Corinne,
Jallu Vincent,
Kaplan Cecile
Publication year - 2007
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2007.01291.x
Subject(s) - neonatal alloimmune thrombocytopenia , genotyping , antigen , immunology , medicine , polymerase chain reaction , serology , platelet membrane glycoprotein , restriction fragment length polymorphism , genotype , fetus , antibody , platelet , pregnancy , biology , genetics , gene
BACKGROUND: Fetal‐neonatal alloimmune thrombocytopenia (FNAIT) linked to rare or private antigens is not a rare event. STUDY DESIGN AND METHODS: Such a case discovered during the follow‐up of a second child with jaundice with mild thrombocytopenia is reported here. Platelet (PLT) genotyping was performed by polymerase chain reaction (PCR)–sequence‐specific primers method and PCR‐restriction fragment length polymorphism (RFLP) analysis. Serologic investigation was done with the monoclonal antibody–specific immobilization of PLT antigens technique. Glycoprotein Ia–specific amplification and sequencing were performed for the polymorphism 807 (exon 7). RESULTS: The mother was found to be HPA‐13aaw, and the father HPA‐13abw. A maternal alloantibody directed against HPA‐13bw has been characterized, leading to the diagnosis of neonatal alloimmune thrombocytopenia. CONCLUSION: This report provides further evidence that NAIT associated with low‐frequency antigens is not restricted to single families. Therefore, laboratory investigation of a suspected case should be carried out in a specialist laboratory well experienced in optimal testing to propose appropriate management for the index case and subsequent pregnancies.