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Evaluation of the COM.TEC cell separator in predicting the yield of harvested CD34+ cells
Author(s) -
Movassaghi Kamran,
Jaques Gabrielle,
SchmittThomssen Angela,
Fischer Eike G.,
Paulus Margit,
Heuft Hans G.,
Schwella Nimord
Publication year - 2007
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2007.01196.x
Subject(s) - tec , separator (oil production) , yield (engineering) , chromatography , chemistry , materials science , physics , composite material , ionosphere , astronomy , thermodynamics
BACKGROUND: This multicenter study was performed with the intention to evaluate the exactness of the predicted CD34+ cell yield calculated by two leukapheresis programs of the cell separator COM.TEC upon the number of donor's circulating CD34+ cells and the blood volume processed. STUDY DESIGN AND METHODS: Patients and healthy donors (n = 166) received mobilization by chemotherapy and/or granulocyte colony‐stimulating factor and underwent CD34+ cell harvest by the leukapheresis programs MNC or RV‐PBSC (n = 203). RESULTS: CD34+ cells were collected by 112 harvests on MNC and by 91 collections on RV‐PBSC. The median collection efficiency of CD34+ cells was significantly better for the program MNC than for RV‐PBSC (p < 0.001): 67% (31‐109) vs. 42% (19‐100). The collected CD34+ cell yield was in median more exactly by MNC than by RV‐PBSC (p < 0.001): 85% (31‐176) vs. 59% (22‐110) of the predicted value. Concentrates obtained by RV‐PBSC showed in median significantly higher percentages of mononuclear cells (p < 0.001) and CD34+ cells (p < 0.001), 86% (43‐99) vs. 56% (25‐95) and 1.2% (0.2‐14.3) vs. 0.4% (0.1‐6.0), and had lower contaminations by erythrocytes (p < 0.001) and platelets (p < 0.001), 13 mL (4‐48) vs. 25 mL (5‐60) and 1.9 × 1011 vs. 3.1 × 1011, than those harvested by MNC. CONCLUSION: The significantly better collection efficiency of CD34+ cells and the more exact prediction of the harvested CD34+ cell yield make the leukapheresis program MNC a safe and efficient procedure. However, concentrates collected by RV‐PBSC are of a better cellular quality with a significantly higher percentage of mononuclear and CD34+ cells and a lower contamination by erythrocytes and platelets.