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Wound‐healing properties of trehalose‐stabilized freeze‐dried outdated platelets
Author(s) -
Sum Ruth,
Hager Sarah,
Pietramaggiori Giorgio,
Orgill Dennis P.,
Dee Josh,
Rudolph Alan,
Orser Cindy,
Fitzpatrick G. Michael,
Ho David
Publication year - 2007
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2007.01170.x
Subject(s) - wound healing , platelet , in vitro , in vivo , andrology , growth factor , chemistry , medicine , surgery , immunology , biology , biochemistry , receptor , microbiology and biotechnology
BACKGROUND: The wound‐healing applications of platelet (PLT)‐derived cytokines, proteins, and membranes is accepted but continues to be investigated. In this study, it is demonstrated that stabilized freeze‐dried PLTs prepared from outdated PLTs (FDPOs) accelerate wound healing and form tube structures as well as stabilized indated freeze‐dried PLTs (FDPIs) and room‐temperature fresh PLTs (RT‐PLTs). STUDY DESIGN AND METHODS: Experiments were designed to compare in vitro and in vivo wound‐healing properties of FDPI, FDPO, and RT‐PLT preparations. The concentration of PLT‐derived growth factor (PDGF)‐ββ and transforming growth factor (TGF)‐β1 was determined, and the abilities of FDPIs, FDPOs and RT‐PLTs to induce endothelial cell proliferation and promote endothelial cell tube formation (cells formed solid spouts connecting neighboring cells to form tube structures) were observed. Wound‐healing characteristics were measured by surgically inducing 1‐cm 2 , full‐thickness wounds on db/db mice (n = 10 per group). The wounds were treated with single or multiple doses of FDPIs and FDPOs. Wound closure rate was determined, and histology samples were evaluated for cellular makeup. RESULTS: FDPOs retained the same levels of PDGF‐ββ and TGF‐β1 and were able to promote endothelial cell proliferation and tube formation in vitro as well as FDPIs or RT‐PLTs. Multiple applications of FDPO accelerated wound closure and enhanced reepithelialization when compared to untreated wounds in db/db mice. CONCLUSION: FDPOs enhanced wound healing in db/db mice as well as FDPIs and RT‐PLTs. Wound closure was obtained 6 days earlier than untreated wounds and histologic examination revealed reduced granulation and increased cellular angiogenesis.