Premium
Leukoreduced buffy coat–derived platelet concentrates photochemically treated with amotosalen HCl and ultraviolet A light stored up to 7 days: assessment of hemostatic function under flow conditions
Author(s) -
Lozano Miguel,
Galan Ana,
Mazzara Roberto,
Corash Laurence,
Escolar Ginés
Publication year - 2007
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2007.01169.x
Subject(s) - buffy coat , ultraviolet , platelet , plateletpheresis , photochemistry , ultraviolet light , medicine , chemistry , surgery , immunology , apheresis , materials science , optoelectronics
BACKGROUND: Amotosalen plus ultraviolet A light photochemical treatment (PCT) inactivates high titers of bacteria, and other pathogens, in platelet concentrates (PCs) potentially allowing the storage of platelets (PLTs) for up to 7 days. Adhesion and aggregation of PLTs to injured vascular surfaces are critical aspects of PLT hemostatic function. STUDY DESIGN AND METHODS: Two ABO‐identical leukoreduced buffy coat–derived PCs in additive solution were mixed and divided: one‐half underwent PCT (PCT‐PCs) and the other was kept as a control (C‐PCs); both were stored under standard conditions. The total number of paired PCs studied was nine. Samples were taken on Day 1 (before PCT) and after 5 and 7 days of storage. The adhesion and aggregation capacities were evaluated under flow conditions in a ex vivo perfusion model. RESULTS: Compared to control, PCT resulted in a decrease in PLT count of 6.5 percent (p = 0.004) and 10.2 percent (p = 0.008) after 5 and 7 days' storage, respectively (n = 9). PLT interaction with subendothelium was mainly in form of adhesion. The surface covered by PCT PLTs on Day 1 was 26.0 ± 4.2 percent (mean ± SEM). On Day 5, PCT‐PCs showed a covered surface of 20.9 ± 2.2 percent, and the C‐PCs, 20.6 ± 1.6 percent. After 7 days, PCT‐PCs produced a nonsignificant higher PLT deposition compared to control (27.1 ± 2.9% vs. 21.2 ± 2.8%, p = 0.06). CONCLUSION: PCT of PCs and storage up to 7 days was associated with a 10.2 percent decrease in PLT count due to processing losses compared to C‐PC. PLT adhesive and aggregating capacities under flow conditions of PCT‐PCs were similar to C‐PCs and remained well preserved for up to 7 days of storage.