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Quantification of nucleated red blood cells in allogeneic marrow graft and impact of processing on recovery
Author(s) -
Marolleau JeanPierre,
Vanneaux Valérie,
Rea Delphine,
Ternaux Brigitte,
Delasse Veronique,
Hubert Valérie,
Chantre Elisabeth,
Traineau Richard,
Robin Marie,
Benbunan Marc,
Socié Gerard,
Larghero Jérôme
Publication year - 2007
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2007.01099.x
Subject(s) - nucleated red blood cell , bone marrow , flow cytometry , cd34 , andrology , haematopoiesis , blood cell , cell , white blood cell , red blood cell , medicine , immunology , stem cell , biology , microbiology and biotechnology , pregnancy , fetus , genetics
BACKGROUND: In allogeneic bone marrow transplantation (BMT), a higher nucleated and CD34+ cell dose has been reported to improve various outcomes. Other cell types, such as lymphocyte subsets, also influenced BMT results. While nucleated red blood cells (NRBCs) represent a subset of bone marrow (BM) cell subpopulation, the question of their quantification in BM grafts and the impact of BM processing on their recovery has not been addressed. STUDY DESIGN AND METHODS: In a prospective study on 77 BM products, NRBCs were enumerated by flow cytometry and the recovery analyzed after manipulation. Because NRBCs could compromise white blood cell count, the impact of NRBC count on CD34+ cell percentage and total nucleated cell (TNC) dose were also determined. RESULTS: The mean percentage of NRBCs in BM grafts was 21.6 percent (range, 7.8%‐40.9%). Mean NRBC recoveries after BM concentration or RBC depletion were 98.4 and 28.7 percent, respectively, close to those obtained for TNC cells (88.6 and 31.3%, respectively). When corrected with NRBC count, the mean percentages of corrected CD34+ cell and TNC dose were significantly modified when compared with uncorrected values, whatever the type of BM manipulation. CONCLUSION: Our data show that NRBC quantification might be of importance to improve quality control of BM products and to evaluate the influence of NRBCs cell dose on outcomes after BMT.