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Monitoring survival and function of transfused platelets in Bernard‐Soulier syndrome by flow cytometry and a cone and plate(let) analyzer (Impact‐R)
Author(s) -
Panzer Simon,
Eichelberger Beate,
Koren Daniela,
Kaufmann Karin,
Male Christoph
Publication year - 2007
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2007.01070.x
Subject(s) - bernard–soulier syndrome , flow cytometry , medicine , platelet , immunology
BACKGROUND: Bernard‐Soulier syndrome (BSS) patients may repeatedly require transfusion of platelets (PLTs). The hemostatic competence of transfused PLTs requires monitoring. STUDY DESIGN AND METHODS: Flow cytometry and a cone and plate(let) analyzer (Impact‐R, DiaMed) were used to monitor survival and function of transfused PLTs in a 7‐year‐old girl with BSS undergoing surgery. Flow cytometry was applied to differentiate autologous PLTs from transfused PLTs by staining for CD42b. The Impact, which measures PLT adhesion and aggregation in response to high shear stress, was used to evaluate PLT function. RESULTS: Transfused PLTs were detectable by flow cytometry for 1 week after transfusion. While the patient’s PLTs did not respond to high shear stress before transfusion, a normal response was documented by the Impact on the day after transfusion and 1 week thereafter. CONCLUSION: Transfused PLTs were detectable by flow cytometry, and their functional activity was demonstrated by the Impact.