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Paired in vitro and in vivo comparison of apheresis platelet concentrates stored in platelet additive solution for 1 versus 7 days
Author(s) -
Shanwell Agneta,
Diedrich Beatrice,
Falker Cecilia,
Jansson Berit,
Sandgren Per,
Sundkvist Lars,
Svensson Leif,
Vesterinen Mervi,
Gulliksson Hans
Publication year - 2006
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2006.00830.x
Subject(s) - plateletpheresis , apheresis , platelet , in vivo , andrology , chemistry , bicarbonate , lactate dehydrogenase , medicine , immunology , biology , biochemistry , enzyme , microbiology and biotechnology
BACKGROUND: To improve clinical access to platelet concentrates (PCs), prolonging the storage period is one alternative, provided that they are free from bacteria. The quality of platelets (PLTs) stored for 1 versus 7 days was compared by in vitro analyses and in vivo recovery and survival in blood donors. STUDY DESIGN AND METHODS: Apheresis PCs from 10 donors were divided and stored in PLT additive solution in 2 equal units for a paired comparison. PLTs in one unit were 111 In‐labeled at 1 day of storage, and PLTs in the other unit were labeled after 7 days of storage. PLTs were injected into the donor after labeling and samples were drawn after 30, 60, and 150 minutes and thereafter once a day for 14 days for recovery and survival measurements. RESULTS: PLT recovery on Day 7 was 80 percent of the recovery on Day 1 (p < 0.05), and the survival on Day 7 was 65 percent of survival on Day 1 (p < 0.005). No significant differences were seen regarding mean PLT volume (MPV), pH, pCO 2 , pO 2 , bicarbonate, or hypotonic shock response. Lactate increased and lactic dehydrogenase increased slightly, whereas glucose and ATP decreased, but not to a critical level. A significant increase in RANTES (110.7 ± 76.6 vs. 277.6 ± 50.8 pg/10 6 PLTs [p < 0.005]) and PLT factor 4 (19.9 ± 9.6 vs. 59.8 ± 7.5 IU/10 6 PLTs [p < 0.0001]) was noticed during storage. CONCLUSION: Recovery and survival of PCs stored for 7 days decreased, but met suggested criteria. Analyzed in vitro parameters showed acceptable results. Randomized patient transfusion studies will provide additional verification of the suitability of 7‐day storage of PLTs.