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Controlled‐rate versus uncontrolled‐rate freezing as predictors for platelet cryopreservation efficacy
Author(s) -
Balint Bela,
Paunovic Dragica,
Vucetic Dusan,
Vojvodic Danilo,
Petakov Marijana,
Trkuljic Miroljub,
Stojanovic Nevenka
Publication year - 2006
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2006.00706.x
Subject(s) - cryopreservation , dimethyl sulfoxide , platelet , flow cytometry , andrology , chemistry , immunology , medicine , biology , embryo , organic chemistry , microbiology and biotechnology
BACKGROUND: Cryobiologic variables responsible for cell injuries and freezing techniques applicable in medical cryopractice should be revised and/or reengineered for minimizing cryoinjuries and maximizing cell recovery. In this study, the efficacy of different cryopreservation protocols based on platelet (PLT) recovery was evaluated. STUDY DESIGN AND METHODS: PLTs (n = 33) were prepared from whole‐blood units. Cell count and viability, PLT morphologic score (PMS), and hypotonic shock response were determined. PLT surface antigens were measured by flow cytometry. Controlled‐rate (with compensated fusion heat) and uncontrolled‐rate freezing methods combined with 6 percent dimethyl sulfoxide were used. RESULTS: PLT recovery was superior in the controlled‐rate setting (91.0 ± 5.5 vs. 86.0 ± 6.5; p < 0.05). PMS was significantly better in controlled‐rate freezing (p < 0.01). GPIb/CD42b expression was reduced in both freezing groups versus control. GP140/CD62p expression was significantly (p < 0.05) lower in the controlled‐rate group and in both frozen groups was significantly higher than in the control groups. CONCLUSION: The use of strictly equalized (1°C/min) controlled‐rate freezing, combined with an intensified cooling rate (2°C/min) during the liquid–to–solid‐phase transition period, allows advanced quantitative and qualitative PLT recovery, even though the minor intergroup differences for some variables were observed.