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HLA‐DRB1 alleles and Jk a immunization
Author(s) -
Reviron Denis,
Dettori Isabelle,
Ferrera Virginie,
Legrand Dominique,
Touinssi Mhammed,
Mercier Pierre,
De Micco Philippe,
Chiaroni Jacques
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2005.04366.x
Subject(s) - genotyping , odds ratio , allele , human leukocyte antigen , immunology , population , polymerase chain reaction , hla drb1 , genetic predisposition , medicine , blood transfusion , biology , genotype , genetics , antigen , gene , environmental health
BACKGROUND: In transfusion medicine, anti‐Jk a has been implicated in hemolytic transfusion reactions. Development of anti‐Jk a after transfusion does not always occur after Jk(a–) patients receive at least 1 unit of Jk(a+) blood unit. This study was designed to identify HLA‐DRB1 alleles associated with predisposition to Jk a immunization after blood transfusion or pregnancy. STUDY DESIGN AND METHODS: Genotyping by polymerase chain reaction and sequence‐specific oligonucleotide probe nonradioactive hybridization/sequence‐specific primers was performed in 20 Jk a ‐immunized patients and 200 controls from the same southern European population. RESULTS: Genotyping showed that HLA‐DRB1*01 was significantly more frequent in Jk a ‐immunized patients than controls: 55 percent versus 17 percent (odds ratio [OR], 5.9; confidence interval [CI], 2.3‐15.5; corrected p value < 0.05). Because HLA‐DRB1*0101, DRB1*0102, and DRB1*1001 share a common sequence in their B1 chain, that is, F in 13, R in 71, and A in 74, HLA genetic predisposition was analyzed by comparing patients and controls with respect to the distribution of F13/R71/A74‐positive and ‐negative alleles. Results demonstrated greater positivity of the F13/R71/A74 sequence (DRB1*0101, *0102, or *1001) in patients than in controls: 65 percent versus 19.5 percent (OR, 7.7; CI, 2.9‐20.5; p < 0.001). CONCLUSION: In conclusion, HLA‐DRB1*0101, DRB1*0102, and DRB1*1001, which share a common DRB1 sequence, appeared to be overrepresented in Jk a ‐immunized patients.