Premium
Microarray‐based genotyping for blood groups: comparison of gene array and 5′‐nuclease assay techniques with human platelet antigen as a model
Author(s) -
Bugert Peter,
McBride Simon,
Smith Graham,
Dugrillon Alex,
Klüter Harald,
Ouwehand Willem H.,
Metcalfe Paul
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2005.04318.x
Subject(s) - genotyping , taqman , snp genotyping , typing , microarray , single nucleotide polymorphism , snp , microbiology and biotechnology , antigen , biology , genotype , gene , computational biology , genetics , polymerase chain reaction , gene expression
BACKGROUND: Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single‐nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins. STUDY DESIGN AND METHODS: By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth‐generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third‐generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA‐1, ‐2, ‐3, ‐5, and‐15 systems. RESULTS: Unambiguous and concordant results were obtained with both techniques for all samples. CONCLUSION: The data presented here validate the use of microarray for large‐scale SNP typing for clinically relevant blood group alloantigens.