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In vitro evaluation of prestorage pooled leukoreduced whole blood–derived platelets stored for up to 7 days
Author(s) -
Heddle N.M.,
Barty R.L.,
Sigouin C.S.,
Boye D.M.,
Nelson E.J.,
Blajchman M.A.,
Kelton J.G.
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2005.04234.x
Subject(s) - platelet , leukoreduction , plateletpheresis , blood product , blood bank , zoology , blood preservation , transfusion medicine , andrology , apheresis , biology , chemistry , food science , immunology , medicine , blood transfusion , surgery , medical emergency
BACKGROUND: Advantages to storing whole blood–derived platelets (PLTs) as a pool for 7 days would include operational efficiencies and facilitation of bacterial testing and pathogen inactivation. The in vitro quality of pre‐storage pooled PLTs stored for up to 7 days was assessed. STUDY DESIGN AND METHODS: Leukoreduced PLTs were pooled before storage (5 units/pool) and stored for either 5 or 7 days. Samples were collected at the time of pooling and either on Day 5 (n = 16‐29) or on Day 7 (n = 4‐30) and tested for biochemical and activation markers and morphology and/or shape change. Control PLTs were stored individually for 5 or 7 days and then tested as indicated above. RESULTS: The mean PLT counts (×10 9 /L) were similar: control PLTs, 1344 (464 SD); and prestorage pooled PLTs, 1327 (220 SD; p = 0.93). On Day 5, the pH value was significantly lower (p ≤ 0.0001) for the prestorage pooled PLTs. Lactate and partial pressure of O 2 (pO 2 ) were significantly higher (p ≤ 0.003). On Day 7 of storage, significant differences were noted with pH, pCO 2 , and hypotonic shock all being lower with the prestorage pooled product, whereas pO 2 , lactate, and morphology scores were higher (p values all < 0.03). Although significant differences were found, the magnitude of the difference would not be expected to impact on transfusion outcomes. CONCLUSION: These results suggest that prestorage pooled PLTs can be stored for up to 7 days; however, studies are needed to ensure that the clinical benefit of PLTs stored as a pool is not inferior to those stored individually.