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Augmented mobilization and collection of CD34+ hematopoietic cells from normal human volunteers stimulated with granulocyte–colony‐stimulating factor by single‐dose administration of AMD3100, a CXCR4 antagonist
Author(s) -
Liles W. Conrad,
Rodger Elin,
Broxmeyer Hal E.,
Dehner Christine,
Badel Karin,
Calandra Gary,
Christensen Jeff,
Wood Brent,
Price Thomas H.,
Dale David C.
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2005.04222.x
Subject(s) - leukapheresis , granulocyte colony stimulating factor , cd34 , haematopoiesis , plerixafor , granulocyte , progenitor cell , medicine , immunology , transplantation , cxcr4 , andrology , stem cell , biology , chemotherapy , microbiology and biotechnology , chemokine , immune system
BACKGROUND: AMD3100, a selective antagonist of CXCR4, rapidly mobilizes CD34+ hematopoietic progenitor cells (HPCs) from marrow to peripheral blood with minimal side effects. STUDY DESIGN AND METHODS: To further investigate potential clinical utility of AMD3100 for CD34+ cell mobilization and collection, a Phase I study in normal volunteers was performed examining single‐dose administration of AMD3100 alone and in combination with a standard 5‐day granulocyte–colony‐stimulating factor (G–CSF) regimen. RESULTS: AMD3100 (160 µg/kg × 1 on Day 5) significantly increased both G–CSF‐stimulated (10 µg/kg/day) mobilization of CD34+ cells (3.8‐fold) and leukapheresis yield of CD34+ cells. Moreover, collection of CD34+ cells was comparable between individuals mobilized by a single‐dose regimen of AMD3100 (240 µg/kg) and individuals mobilized with a 5‐day regimen of G–CSF. AMD3100‐mobilized leukapheresis products contained significantly greater numbers of T and B cells compared to G–CSF‐stimulated leukapheresis products. CONCLUSION: These findings indicate that AMD3100 can be used alone or as an adjunct to G–CSF to mobilize cells for HPC transplantation.